纯化混合胰岛素/胰岛素样生长因子受体胰岛素样生长因子结合,但不是胰岛素,具有高亲和力。
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秀妈,CE, Siddle K
纯化混合胰岛素/胰岛素样生长因子受体胰岛素样生长因子结合,但不是胰岛素,具有高亲和力。
3月1;j . 1993 290 (Pt 2): 419 - 26所示。
- PubMed ID
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8452530 (在PubMed]
- 文摘
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混合胰岛素/胰岛素样生长因子受体(IGF-I)曾被描述在人类胎盘,但却不可能去研究它们的属性在古典胰岛素受体和I型IGF受体。促进混合动力车的净化,我们产生了anti-peptide单克隆抗体IGFR 1 - 2,针对c端肽的I型IGF受体beta-subunit。抗体绑定本地人类和老鼠的I型IGF受体,和反应特别beta-subunit免疫印迹。随着胎盘古典类型的微粒体膜被耗尽我IGF受体通过孵化一个固定单克隆抗体IGFR 24-55,这反应与I型受体与混合受体却很差。剩余混合受体被孤立的孵化与固定化抗体IGFR 1 - 2,并恢复了洗脱合成肽抗原过剩。绑定属性的混合动力车相比immuno-affinity-purified胰岛素受体和I型IGF受体,通过使用放射性配体125 i-igf-i和125 i-insulin。混合动力车约。20倍(约125 125 i-igf-i i-insulin示踪剂浓度。0.1海里)。125 i-insulin绑定,但不是125 i-igf-i,混合动力车与二硫苏糖醇治疗后增加到减少二硫化物alpha-subunits之间的债券。 Hybrids behaved very similarly to type I receptors with respect to the inhibition of 125I-IGF-I binding by unlabelled IGF-I and insulin. By contrast, the affinity of hybrids for insulin was approx. 10-fold lower than that of classical insulin receptors, as assessed by inhibition of 125I-insulin binding by unlabelled hormone. It is concluded that the properties of insulin receptors, but not IGF receptors, are markedly affected by assembly as hybrid compared with classical structures, and that hybrids are more likely to be responsive to IGF-I than insulin under physiological conditions.