一氧化氮合酶表达的神经元对碘氧基苯甲醚在人类视网膜在低水平。
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公园CS,克里希纳吉C,公园R, G
一氧化氮合酶表达的神经元对碘氧基苯甲醚在人类视网膜在低水平。
细胞摩尔一般人。1996年8月,16 (4):499 - 515。
- PubMed ID
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8879752 (在PubMed]
- 文摘
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1。的表达一氧化氮合酶的神经元对碘氧基苯甲醚(nNOS)在人类视网膜组织学习。人类视网膜的cDNA序列克隆聚(A) + RNA的rt - pcr方法,包含4302个基点的开放框架编码1434个氨基酸。这个序列显示遗传多态性的可能性相比,人类大脑的形式。2。限制片段长度多态性(RFLP)模式的部分cDNA片段显示,有遗传多态性观察神经元NOS。重要的差别在一定地区人类视网膜和大脑之间的表格。这个地区是框架的结果转变的三个胞。在这项研究中,从人类大脑区域(小脑)和骨骼肌以及视网膜测序证实这个地区的差异。这些组织的序列是完全相同的。这表明nNOS基因的遗传多态性是由于单碱基替换而不是添加或删除帧转移现象的基础。 3. The nNOS mRNA of approximately 12 kb was detected by northern blot analysis. The lower level of the expression was distinguished in comparison to those of human brain and skeletal muscle. The cDNA transiently transfected into CHO-K1 cells expressed a protein which contained a significant level of NOS activity. The size of the nNOS was found to be approximately 160 kDa by both in vitro and in vivo translation systems. This NOS was calcium dependent and the K(m) for arginine was 4.4 microM. 4. The Ca+2, L-arginine and NADPH dependency along with the inhibitory effect of N-nitro-L-arginine on NOS activity were evaluated. The finding of a constitutive from of NOS in human retina, which is calcium-NADPH dependent, gives further credence to the possible role of nitric oxide in retinal function and neuronal diseases.