的规定MS-KIF18A与雌激素受体表达和相声。
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Zusev M, Benayahu D
的规定MS-KIF18A与雌激素受体表达和相声。
《公共科学图书馆•综合》。2009年7月28日,4 (7):e6407。doi: 10.1371 / journal.pone.0006407。
- PubMed ID
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19636373 (在PubMed]
- 文摘
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这项研究提供了一个新颖的观点之间的交互MS-KIF18A,驱动蛋白的蛋白质,雌激素受体α(ERalpha)的体内和体外研究。此外,MS-KIF18A表达的调节雌激素研究在基因和蛋白水平。一个重组蛋白之间的联系;ERalpha和MS-KIF18A拉下分析了体外。这样的相互作用也被证明是内源性蛋白质MBA-15细胞被发现在细胞质中,雌激素是上调。此外,这些蛋白质与转录因子NF-kappaB被确认。MS-KIF18A mRNA表达测定体内与年龄和雌激素水平在小鼠和大鼠模型。减少MS-KIF18A mRNA水平测定在老OVX-estrogen枯竭的老鼠比年轻的动物。低MS-KIF18A mRNA表达OVX大鼠被雌激素治疗恢复。我们研究的规定MS-KIF18A由雌激素使用荧光素酶报告基因的转录和染色质immuno-precipitation(芯片)化验。 The luciferase reporter gene assay demonstrated an increase in MS-KIF18A promoter activity in response to 10(-8) M estrogen and 10(-7)M ICI-182,780. Complimentary, the ChIP assay quantified the binding of ERalpha and pcJun to the MS-KIF18A promoter that was enhanced in cells treated by estrogen and ICI-182,780. In addition, cells treated by estrogen expressed higher levels of MS-KIF18A mRNA and protein and the protein turnover in MBA-15 cells was accelerated. Presented data demonstrated that ERalpha is a defined cargo of MS-KIF18A and added novel insight on the role of estrogen in regulation of MS-KIF18A expression both in vivo and in vitro.