人类基因编码tryptophanyl-tRNA合成酶:干扰素反应元素和exon-intron组织。
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杰AY, Frolova LY Sudomoina妈,Kisselev噢
人类基因编码tryptophanyl-tRNA合成酶:干扰素反应元素和exon-intron组织。
基因。1993年6月30日,128 (2):237 - 45。
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7685728 (在PubMed]
- 文摘
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最近,我们克隆和测序的互补编码人类tryptophanyl-tRNA合成酶(hWRS)[基因Frolova et al ., 109 (1991) 291 - 296]。独立,它已经表明,这种蛋白质是由干扰素(IFN)γ和阿尔法(Fleckner et al ., Proc。国家的。学会科学。美国88 (1991)11520 - 11524;鲁宾et al .,生物。化学,266 (1991)24245 - 24248]。管家酶的这种不同寻常的特性提出了基因是如何管理的问题。因为目前的基因组结构hWRS是未知的,这个问题仍然没有解决。这里,exon-intron hWRS组织已被破译。该基因由至少12个外显子,跨度超过35 kb的DNA。 At least two alternative noncoding exons precede ten coding exons. Upstream from the first exon, two GGAAAN(N/-)GAAA sequences, which are considered to be IFN-stimulating response elements (ISRE), have been revealed. The same consensus was also found in the intron region in close vicinity to the 5' end of the second exon. Thus, the IFN-stimulated synthesis of hWRS is presumably due to gene activation at the transcriptional level. Alignment of hWRS amino acid sequences has shown that exons V to XI of hWRS encode regions of structural similarity with bacterial WRS, whereas the N-terminal portion of the protein encoded by exons II to IV exhibits no homology with bacterial WRS.(ABSTRACT TRUNCATED AT 250 WORDS)