克隆人类的互补编码线粒体和胞质丝氨酸hydroxymethyltransferases和染色体定位。
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Garrow助教,布兰诺AA, Whitehead VM,陈XN邓肯•RG Korenberg JR,肖恩·B
克隆人类的互补编码线粒体和胞质丝氨酸hydroxymethyltransferases和染色体定位。
生物化学杂志。1993年6月5日;268 (16):11910 - 6。
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8505317 (在PubMed]
- 文摘
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人类的互补为胞质和线粒体丝氨酸hydroxymethyltransferase (SHMT)被克隆功能互补的大肠杆菌glyA突变与人类cDNA图书馆。的互补脱氧胞质酶编码483 - 53020 (r)的残留蛋白质。线粒体酶的互补编码一个成熟蛋白质的474 52400 (r)的残留。推断蛋白质序列有着高度的序列彼此身份(63%),和个人同功酶是高度同源类似兔肝胞质(92%身份)和线粒体(97%身份)SHMT同功酶(马提尼,F。、Angelaccio年代。、Pascarella年代。Barra D。之旅,F。,Schirch诉(1987)生物。化学。262年,5499 - 5509;马提尼酒,F。马拉什,B。Tanci, P。、Angelaccio年代。、Pascarella年代。Barra D。之旅,F。,Schirch诉(1989)生物。264年化学,8509 - 8519)。 SHMT is a highly conserved protein with the human isozymes retaining about 43% sequence identity with the E. coli protein. The human cytosolic and mitochondrial SHMT genes were localized to chromosome regions 17p11.2 and 12q13, respectively. The high degree of nucleotide sequence identity between the two isozymes, and the presence of keratin genes in both chromosomal regions, is consistent with these regions of chromosome 12 and 17 arising by a duplication event.