识别的二糖(Xyl-Glc)和三糖(Xyl2-Glc) O-glycosidically与丝氨酸残基的表皮生长因子以人为因素第七和第九域蛋白质Z和牛Z。
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Nishimura H,川端康成,Kisiel W,哈泽年代,Ikenaka T, T高雄,Shimonishi Y, Iwanaga年代
识别的二糖(Xyl-Glc)和三糖(Xyl2-Glc) O-glycosidically与丝氨酸残基的表皮生长因子以人为因素第七和第九域蛋白质Z和牛Z。
J生物化学杂志。1989年12月5日,264 (34):20320 - 5。
- PubMed ID
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2511201 (在PubMed]
- 文摘
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我们最近描述了一个独特的三糖与丝氨酸残基的表皮生长因子以域牛凝血因子VII (Ser-52)和第九(Ser-53)(哈泽,S。川端康成,S。西村,H。Takeya, H。Sueyoshi, T。基金经理人,T。、Iwanaga年代。高雄,T。Shimonishi, Y。Ikenaka, t . j .生物化学(1988)。(东京)104年,867 - 868)。糖链中标识这些凝血因子由1摩尔的己糖(葡萄糖(相关)和2摩尔的戊糖(木糖(Xyl]。我们报告,人力因素第七和第九和蛋白质Z和牛Z等也含有碳水化合物与半个丝氨酸残基在同一位置中发现牛因素第七和第九。糖肽来源于这些蛋白质受到糖氨基酸序列和组件分析和快原子轰击质谱分析。 The results indicate that the glycopeptide derived from human factor IX contains 1 mol each of Glc and Xyl. The reducing end of this disaccharide was identified as Glc by analyzing the disaccharide generated by hydrazinolysis. In contrast, human factor VII and protein Z yielded two different glycopeptides which contained Glc and Xyl at molar ratios of 1:1 and 1:2, respectively, suggesting microheterogeneity of these O-linked sugar chains. Bovine protein Z glycopeptide contained 1 mol of Glc and 2 mol of Xyl. These sugar compositions were confirmed by analyses of the intact proteins. In relation to the trisaccharide sugar chain previously discovered in bovine factors VII and IX, these findings indicate the existence of a Xyl2-Glc-Ser and a Xyl-Glc-Ser structure in the first epidermal growth factor-like domains of human factors VII and IX and protein Z in addition to that of bovine protein Z. Whether these carbohydrate moieties contribute to the biological activities of these proteins is unknown.