分子表征两个小说人类降钙素受体的亚型。
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Balasubramanian Beaudreuil J,年代,Chenais J, Taboulet J, Frenkian M, Orcel P, Jullienne,霍恩WC, de Vernejoul MC Cressent M
分子表征两个小说人类降钙素受体的亚型。
基因。2004年12月8日,343 (1):143 - 51。
- PubMed ID
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15563840 (在PubMed]
- 文摘
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降钙素抑制骨吸收作用于破骨细胞通过特定的受体。降钙素受体(CTR)还发现在许多其他正常和恶性组织和细胞系。它已经在几个物种包括人类克隆和测序。它属于一个子类seven-transmembrane G protein-coupled受体。四个人类CTR (H-CTR)亚型或者拼接产生的mRNA此前就已被描述。两个H-CTR编码dna包含一个身份不明的50个基点插入现在报告T47D细胞。50个基点插入对应于一个DNA区域位于第9外显子和外显子10之间,而且似乎来自一个可变剪接过程。274年和290年两个H-CTR互补编码aa长亚型。两者都从公认的第四跨膜域C-tail中删除。他们存在不同的(H-CTR5)与否(H-CTR6)之前所知16-aa插入的公认的第一个细胞内循环。 Cell- and tissue-distribution analysis using RT-PCR demonstrates that the shorter one, HCTR6, is more prevalent. The mRNA of both isoforms was detected in giant cell tumor, whereas only H-CTR6 mRNA was detected in TT cells and kidney tissue. Neither H-CTR5 nor H-CTR6 could be detected in peripheral blood mononuclear cells cultured in the presence of RANKL, in MCF7 cells, and in cortical brain and ovarian tissues. When H-CTR6 was transiently expressed in HEK293 cells, CT failed to induce production of cAMP or to bind to the receptor. These suggest either an intrinsic loss of ligand binding function, or an altered intracellular trafficking. Our findings therefore indicate the existence of two novel splice variants of the H-CTR and confirm that multiple splicing patterns could be involved in the post-transcriptional regulation of the gene.