[米非司酮的失败引起的中断怀孕:点突变在人类基因密码子722孕激素受体基因)。
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程高Y, L,刘Y
[米非司酮的失败引起的中断怀孕:点突变在人类基因密码子722孕激素受体基因)。
陈中华福柯9月杂志。1998;33 (9):549 - 52。
- PubMed ID
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10806733 (在PubMed]
- 文摘
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目的:检测激素结合域的点突变(HBD)人类的孕激素受体基因(hPR)通过分子生物学方法在女性与米非司酮不敏感。方法:26岁女性5医院同意了这项研究。16名女性被研究小组:继续妊娠11和5稽留流产。10与成功的堕胎的妇女作为对照组。蜕膜组织收集每个女人和RNA提取和纯化。逆转录-聚合酶链反应(rt - pcr)、单链构象多态性(SSCP)和限制性内切酶反应进行。所有女性随访。结果:没有HBD hPR的基因的点突变在对照组发现除了一个点突变的痕迹。HBD hPR的基因的点突变检测在7的女性继续怀孕。米非司酮在midluteal阶段管理两个妇女和没有观察阴道出血。 No mutation of hPR gene in the other women with continuing pregnancy. Three women had pregnancy again in 6 months and had successful abortion. The other one administrated mifepristion in midluteal phase and had second vaginal bleeding. No point mutation of hPR gene was detected in women with missed abortion. CONCLUSION: There is point mutation of HBD of hPR gene in women with insensitivity to mifepristone, and women with point mutation of hPR gene detected by molecular biological methods are really insensitive to mifepristone.