PSM-E的高表达与肿瘤相关年级在前列腺癌:一个新的或者拼接前列腺特异性膜抗原的变异。
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曹肯塔基州,毛泽东XP,王DH,徐L,元《GQ》,戴平方,郑BJ,邱SP
PSM-E的高表达与肿瘤相关年级在前列腺癌:一个新的或者拼接前列腺特异性膜抗原的变异。
前列腺癌。2007年12月1;67 (16):1791 - 800。
- PubMed ID
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17929272 (在PubMed]
- 文摘
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背景:前列腺特异性膜抗原(PSMA)在前列腺癌(PCa)已针对PCa的治疗和诊断。在最近的研究中,PSMA cDNA被rt - pcr克隆从PCa组织。测序后,一个新的拼接的变体(PSM-E)及其特异性发现PSMA PCa的评估。方法:以PSM-E和mRNA PSMA LNCaP,曲泽,前列腺或nonprostatic恶性肿瘤。转染后与PSM-E cDNA曲泽pcDNA3.0向量,PSM-E表达测定免疫荧光和免疫印迹。PSM-E和PSMA mRNA水平量化实时PCR试验在正常前列腺(n = 7),良性前列腺增生(BPH) (n = 22)和PCa (n = 41)。年级水平和肿瘤之间的相关性进行了分析。结果:PSM-E cDNA PSMA除了97 -核苷酸相同地区和93核苷酸地区。PSM-E mRNA PSMA被发现在PCa和LNCaP,不在曲泽;可以发现在一些nonprostatic PSMA肿瘤而PSM-E不是。 The expression of PSM-E protein was detected in transfected cells. Significant difference of PSM-E mRNA levels was observed among normal prostate, BPH and PCa (P < 0.001), and PSM-E levels increased with increasing Gleason score (r = 0.514, P < 0.001). PSMA mRNA levels were higher in BPH and PCa than in normal prostate (P < 0.001), but no difference between BPH and PCa, no significant correlation was observed between PSMA levels and Gleason score (r = 0.229, P = 0.057). CONCLUSIONS: PSM-E may be a potential prognostic indicator for PCa progression and may be a new target antigen for therapy of PCa.