人类的雌激素受体β结合DNA的方式类似,与雌激素受体α二聚。
文章的细节
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引用
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速度P,泰勒J, Suntharalingam年代,库姆斯RC,阿里
人类的雌激素受体β结合DNA的方式类似,与雌激素受体α二聚。
生物化学杂志。1997年10月10日;272 (41):25832 - 8。
- PubMed ID
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9325313 (在PubMed]
- 文摘
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小说的克隆雌激素受体β(ERbeta表示)最近被描述(柯伊伯·g·g·j·M。Enmark E。Pelto-Huikko, M。尼尔森,S。,Gustafsson J-A。(1996)Proc。国家的。学会科学。美国93年,5925 - 5930年和Mosselman, S。,j·波尔曼和Dijkema r(1996) 2月。392年,调查)。ERbeta高度同源的“古典”雌激素受体α(这里称为ERalpha),已经被证明可以结合雌激素亲和力ERalpha相似,并激活报告基因的表达含有雌激素响应estrogen-dependent地元素。这里我们描述功能研究比较人类的DNA结合能力ERalpha化验和β在凝胶转变。我们表明,DNA结合ERalpha和β也同样受到高温的影响没有配体或17 beta雌二醇和局部雌激素受体激动剂4-hydroxy-tamoxifen。 In the absence of ligand, DNA binding by ERalpha and beta is rapidly lost at 37 degrees C, while in the presence of 17beta-estradiol and 4-hydroxy-tamoxifen, the loss in DNA binding at elevated temperature is much more gradual. We show that the loss in DNA binding is not due to degradation of the receptor proteins. However, while the complete antagonist ICI 182, 780 does not "protect" human ERalpha (hERalpha) from loss of DNA binding at elevated temperature in vitro, it does appear to protect human ERbeta (hERbeta), suggestive of differences in the way ICI 182, 780 acts on hERalpha and beta. We further report that ERalpha and beta can dimerize with each other, the DNA binding domain of hERalpha being sufficient for dimerization with hERbeta. Cell and promoter-specific transcription activation by ERalpha has been shown to be dependent on the differential action of the N- and C-terminal transcription activation functions AF-1 and AF-2, respectively. The existence of a second estrogen receptor gene and the dimerization of ERalpha and beta add greater levels of complexity to transcription activation in response to estrogens.