人类alpha2-macroglobulin由多个域预测的同源性与补充组件C3。
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Doan N,捞到PG
人类alpha2-macroglobulin由多个域预测的同源性与补充组件C3。
j . 2007 10月1日,407 (1):23-30。
- PubMed ID
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17608619 (在PubMed]
- 文摘
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人类alpha2M (alpha2-macroglobulin)和补体C3和C4组件硫醇ester-containing蛋白质进化从同一祖先的基因。最近的结构测定人类C3允许的详细预测人类alpha2M域内的位置。我们描述这三个α的表达和描述(2)M的稳定域预测参与硫醇酯在本机alpha2M和激活的诱饵蛋白水解作用。表达的三个新域MG2(巨球蛋白域2),泰德(硫醇ester-containing域)和幼崽(补充蛋白质子组件C1r / c1,海胆胚胎生长因子和骨形态形成蛋白1)域。与之前描述RBD(受体结合域),他们代表约。alpha2M多肽的42%。他们的表情折叠域alpha2M强烈支持预测域组织。x射线晶体结构MG2显示它有纤连蛋白3型脊髓灰质炎病毒引起褶皱的类似于MG1-MG8 C3。泰德是,正如预测的那样,一个α螺旋域。幼崽拼接领域由两段侧面TED的多肽的主要结构。 In intact C3 TED interacts with RBD, where it is in direct contact with the thiol ester, and with MG2 and CUB on opposite, flanking sides. In contrast, these alpha2M domains, as isolated species, show negligible interaction with one another, suggesting that the native conformation of alpha2M, and the consequent thiol ester-stabilizing domain-domain interactions, result from additional restraints imposed by the physical linkage of these domains or by additional domains in the protein.