的敏捷domain-containing soc箱蛋白1 (SSB-1)与满足,提高肝细胞生长因素Erk-Elk-1-serum响应元件通路。
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王,李Z,扰乱他们,吴G
的敏捷domain-containing soc箱蛋白1 (SSB-1)与满足,提高肝细胞生长因素Erk-Elk-1-serum响应元件通路。
J生物化学杂志。2005年4月22日,280 (16):16393 - 401。Epub 2005年2月15日。
- PubMed ID
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15713673 (在PubMed]
- 文摘
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抑制细胞因子信号(soc)蛋白家族包括敏捷(重复在解放军和RyR) domain-containing soc箱蛋白质(单边带)亚科,由四个成员,SSB-1, SSB-2 SSB-3, SSB-4。这些蛋白质包含中央敏捷领域和c端soc箱。虽然有些soc蛋白质亚科的函数作为一个大家庭适配器ubiquitin-protein isopeptide连接调节某些信号通路,单边带亚科的功能还有待确定。在先前的研究中,我们发现两个敏捷domain-containing蛋白质,RanBP9 RanBP10,与通过敏捷领域。在目前的研究中,我们探索了单边带的功能蛋白的调节肝细胞生长因子(HGF)遇到的信号。我们的结果表明,所有四个单边带蛋白质也与满足。交互会见了SSB-1进一步调查。我们证明SSB-1必然会遇到酪氨酸激酶域通过其活泼的域。互动会见SSB-1缺失和HGF的存在,但胶质瘤治疗导致更多SSB-1的招聘。我们表明,过度的SSB-1但不是其他单边带蛋白增强HGF-induced血清反应元素(行为)荧光素酶的活动。 Overexpression of SSB-1 exhibited no effect on the basal level or epidermal growth factor-induced SRE-luciferase activity. SSB-1 also enhanced HGF-induced Erk phosphorylation. Suppression of SSB-1 by the RNA interference method down-regulated HGF-induced SRE-luciferase activity and decreased Elk-1 activation. These results suggest that SSB-1 may play an important role in enhancing the HGF-induced Erk-Elk-1-SRE pathway. Furthermore, we demonstrated that in response to HGF stimulation, the SSB-1 protein became phosphorylated at tyrosine residue 31. The phosphorylated SSB-1 protein bound to p120Ras-GTPase-activating protein (GAP) but did not promote the degradation of p120RasGAP, indicating that enhanced HGF-MET signaling by overexpression of SSB-1 was not dependent on p120RasGAP degradation.