与c-Jun n -末端激酶相互作用蛋白结构同源的新型人精子相关抗原9 (SPAG9)的鉴定
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Jagadish N, Rana R, Selvi R, Mishra D, Garg M, Yadav S, Herr JC, Okumura K, Hasegawa A, Koyama K, Suri A
与c-Jun n -末端激酶相互作用蛋白结构同源的新型人精子相关抗原9 (SPAG9)的鉴定
生物化学学报，2005;32(1):73-82。
- PubMed ID
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15693750 (PubMed视图]
- 摘要
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我们报道了一种新的精子相关抗原9 (SPAG9)蛋白与JNK (c-Jun n -末端激酶)相互作用蛋白3具有结构同源性。SPAG9是一个位于人类染色体17q21.33上的单拷贝基因，与小鼠11号染色体的位置一致，早前被证明只在睾丸中表达[Shankar, Mohapatra和Suri (1998) Biochem]。Biophys。参考文献。243,561-565]。SPAG9氨基酸序列分析显示其与jnk结合结构域一致，并预测其为盘绕结构域、亮氨酸拉链结构域和跨膜结构域。二级结构分析预测SPAG9为α -螺旋结构，并经CD谱证实。用串联质谱对重组SPAG9高阶聚集体进行微测序，证实其氨基酸序列和单原子质量为83.9 kDa。对SPAG9及其缺失突变体的瞬时表达表明，具有扩展盘绕结构域的亮氨酸拉链和SPAG9的跨膜结构域都是二聚化和正确定位所必需的。对MAPK(丝裂原活化蛋白激酶)相互作用的研究表明，与JNK1相比，SPAG9与JNK3和JNK2具有更高的结合亲和力。未观察到与p38 α或细胞外信号调节激酶途径的相互作用。 Polyclonal antibodies raised against recombinant SPAG9 recognized native protein in human sperm extracts and localized specifically on the acrosomal compartment of intact human spermatozoa. Acrosome-reacted spermatozoa demonstrated SPAG9 immunofluorescence, indicating its retention on the equatorial segment after the acrosome reaction. Further, anti-SPAG9 antibodies inhibited the binding of human spermatozoa to intact human oocytes as well as to matched hemizona. This is the first report of sperm-associated JNK-binding protein that may have a role in spermatozoa-egg interaction.