CD40-tumor坏死因子receptor-associated因子(TRAF)交互:CD40的监管信号通过多个TRAF结合位点和TRAF hetero-oligomerization。
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普伦党卫军,米勒HG, Everdeen DS,见鬼TT, Crute JJ, Kehry先生
CD40-tumor坏死因子receptor-associated因子(TRAF)交互:CD40的监管信号通过多个TRAF结合位点和TRAF hetero-oligomerization。
生物化学。1998年8月25日,37 (34):11836 - 45。
- PubMed ID
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9718306 (在PubMed]
- 文摘
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CD40是肿瘤坏死因子受体超家族成员,提供激活信号在B细胞等抗原递呈细胞,巨噬细胞和树突细胞。Multimerization CD40配体的启动信号通过招募TNF receptor-associated因素(TRAFs) CD40胞质域。重组人类TRAF蛋白在昆虫细胞生化反应特点和用于精细地图TRAF绑定地区人类CD40胞质域。TRAF1、TRAF2 TRAF3 TRAF6,但不是TRAF4或TRAF5,直接绑定到CD40胞质域。CD40与TRAF2的互动和TRAF3强于TRAF1和TRAF6的交互。全身TRAF3 TRAF5 hetero-oligomers形成,大概是通过预测异亮氨酸拉链。TRAF3-TRAF5 hetero-oligomers与CD40,表明TRAF5可以间接招募CD40胞质域。重叠的多肽合成纤维素膜用于地图上每个TRAF交互区域。TRAF1、TRAF2 TRAF3与同一地区。识别网站TRAF6膜近端是不重叠的区域。 Using peptides with progressive deletions, a minimal TRAF1, TRAF2, and TRAF3 binding region was mapped to the PVQET sequence in the CD40 cytoplasmic domain. The minimal region for TRAF6 binding was the sequence QEPQEINF. These studies demonstrate that the CD40 cytoplasmic domain contains two nonoverlapping TRAF binding regions and suggest that TRAF1, TRAF2, and TRAF3 could bind competitively to one site. Relative affinities and competition of individual and hetero-oligomeric TRAF proteins for CD40 binding sites may contribute to receptor specificity and cell-type selectivity in CD40-dependent signaling.