人类dTMP激酶:基因表达和酶活性与细胞周期进程和细胞生长。
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黄SH,唐,Drisco B,张平方,西格R,李C,郑大世
人类dTMP激酶:基因表达和酶活性与细胞周期进程和细胞生长。
DNA细胞杂志。1994;13 (5):461 - 71。
- PubMed ID
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8024690 (在PubMed]
- 文摘
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dTMP磷酸化的激酶(E.C.2.7.4.9)催化dTMP对应的二磷酸。这种酶对DNA合成体内至关重要,是一个重要的中间酶在许多嘧啶模拟药物的途径。在这个报告中,我们描述了人类dTMP激酶基因的隔离功能互补的酿酒酵母细胞周期突变,cdc8。互补脱氧核糖核酸序列显示一个开放阅读框,编码一个蛋白质的分子量23806。推断蛋白质序列与已知dTMP激酶从不同的生物序列。虽然功能互补和结构守恒的,表达了人类dTMP激酶在酵母显示小酶活性。相比之下,人类dTMP激酶活性可以从基因克隆到杆状病毒表达系统表达,就是明证酶活性增加了4到5倍。与酵母dTMP激酶,人类dTMP激酶不含半胱氨酸残基后守恒glycine-rich循环,但其酶活性仍含巯基的抑制剂的影响,5、5 ' -dithio-bis (2-nitrobenzoic酸)(DTNB)。dTMP激酶mRNA水平及其酶活性在细胞周期波动,在S期达到高峰。因此,像酿酒酵母CDC8(编码dTMP激酶),人类的相同器官mRNA和酶活性也细胞周期调控。 We have also examined four neuroblastoma cell lines for dTMP kinase mRNA levels and its kinase activities, which appear to vary according to cell growth rate. Our results suggest that the expression of the dTMP kinase gene and its activity coincide with various stages of cell growth. The identification of the human dTMP kinase gene and expression of its product in the baculovirus expression system should facilitate study of the mechanism of gene regulation and its role in pyrimidine metabolism.