产品形成和phosphoglucomutase活动Lactococcus lactis:小说phosphoglucomutase基因的克隆和表征。
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钱N,斯坦利GA,缩聚,Radstrom P
产品形成和phosphoglucomutase活动Lactococcus lactis:小说phosphoglucomutase基因的克隆和表征。
微生物学。1997年3月,143 (Pt 3): 855 - 65。
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9084169 (在PubMed]
- 文摘
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麦芽糖代谢Lactococcus lactis包括转换奶到1-phosphate葡萄糖- 6 -磷酸,反应是一个可逆催化了maltose-inducible和glucose-repressible beta-phosphoglucomutase (beta-PGM)。该基因编码beta-PGM (pgmB)从基因组文库克隆l . lactis使用抗体。5695个基点的核苷酸序列片段决心和6个orf,包括pgmB基因被发现。计算基因表达的多肽分子质量24210 Da,在协议的分子质量纯化beta-PGM (kDa 25日)。短序列的n端被发现类似于已知metal-binding域。的表达在L beta-PGM lactis被发现被海藻糖和蔗糖诱导也,乳糖和压抑的生长介质。这表明beta-PGM不完全降解麦芽糖,但它还参与其他碳水化合物的代谢。beta-PGM的具体活动的麦芽糖浓度在发酵过程中依赖于媒介。的最大beta-PGM特定活动增加了4.6倍,和特定的增长7倍,当麦芽糖浓度从0.8到11.0 g l - 1。此外,更高的乳酸生产相对于甲酸,乙酸和乙醇观察初始麦芽糖浓度介质时增加。 The specific activity of alpha-PGM responded similarly to beta-PGM, but the magnitude of the response was lower. Preferential sugar utilization and alpha- and beta-PGM suppression was observed when L. lactis was grown on the substrate combinations glucose and maltose, or lactose and maltose; maltose was the least-preferred sugar. In contrast, galactose and maltose were utilized concurrently and both PGM activities were high throughout the fermentation.