分子加料对鸟类繁殖的影响。

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尤德CA,格雷厄姆JK,米勒

分子加料对鸟类繁殖的影响。

幼禽Sci。2006年7月,85 (7):1285 - 93。

PubMed ID

16830870 (在PubMed

]

文摘

加料(NCZ)是一种anticoccidial药物通常用于家禽行业可以负面影响繁殖减少产蛋,蛋重,蛋的孵化率。NCZ影响生殖的分子机制是未知的。脂蛋白脂肪酶,卵黄蛋白原、转谷氨酰胺酶和钙都是参与蛋形成和胚胎发生。因此,在体外实验中被用来评估4潜在的作用机制NCZ鸡蛋形成和胚胎发生。首先,脂蛋白脂肪酶进行分析来确定NCZ增加脂蛋白脂肪酶活性。第二,卵黄蛋白原磷酸化是评估来确定NCZ充当卵黄蛋白磷酸酶。第三,转谷氨酰胺酶活性测定来确定NCZ抑制转谷氨酰胺酶的活动。最后,公牛精子被用作模型来确定具体的可以被NCZ channel-mediated钙吸收。加料增加脂蛋白脂肪酶的活性在体外的3.9和7.8 microg NCZ /毫升。加料公牛精子细胞内钙含量增加,这表明它还充当一个钙离子载体。 The portion of the NCZ molecule responsible for the increase in intracellular calcium is 2-hydroxy-4,6-dimethylpyrimidine. Nicarbazin affected vitellogenin phosphorylation but only at a concentration many times higher than expected plasma values. Nicarbazin also inhibited transglutaminase activity in vitro. Whereas the 4,4'-dinitrocarbanilide portion of the NCZ molecule inhibited transglutaminase activity, the 2-hydroxy-4,6-dimethylpyrimidine portion increased transglutaminase activity. All of these assays were conducted in vitro; therefore these results should be viewed as preliminary findings to aid in directing further research on the effect of NCZ on reproduction in vivo. Because NCZ increases lipoprotein lipase activity and acts as a calcium ionophore, future experiments should investigate these effects in particular.

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药物

加料