隔离和表征14.5 -kda trichloroacetic-acid-soluble平移抑制剂蛋白质从人类单核细胞,调节细胞分化。
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Schmiedeknecht G、C Kerkhoff Orso E, Stohr J, Aslanidis C,纳吉通用、施密茨Knuechel R, G
隔离和表征14.5 -kda trichloroacetic-acid-soluble平移抑制剂蛋白质从人类单核细胞,调节细胞分化。
。1996欧元12月1;242 (2):339 - 51。
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8973653 (在PubMed]
- 文摘
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14.5 trichloroacetic-acid-soluble -kda蛋白(p14.5)已经从人类分离单核吞噬细胞(MNP)三氯乙酸提取、制备电泳和疏水亲和色谱法;五胰蛋白酶的肽受到蛋白质测序。蛋白的全长cDNA克隆和测序λgt11人类肝脏图书馆。互补脱氧核糖核酸表现出惊人的相似,一只老鼠蛋白优先表达肝细胞和肾小管上皮细胞。编码的蛋白质长137个氨基酸,类似于小蛋白质的新假设的家人目前未知函数,名为YER057c / YJGF。人类重组p14.5体外抑制蛋白质合成在一个兔网织红细胞溶解产物系统。与其他蛋白质合成抑制剂,p14.5不是磷酸化尽管公认的磷酸化网站的存在。p14.5 mRNA在新鲜分离的单核细胞弱表达,但明显调节当这些单核细胞进行分化。这也反映在differentiation-dependent增加蛋白质浓度从胞质分数通过免疫印迹和fluorescence-activated permeabilized细胞的流式细胞术。differentiation-dependent mRNA和蛋白表达p14.5进一步提出一个低表达的观察各种肝脏和肾脏的肿瘤细胞和高表达在评估完全分化的细胞免疫组织化学和北部的印迹。 The highest mRNA expression was found in hepatocytes and renal distal tubular epithelial cells and only weak expression was found in other human tissues as evaluated by northern blot analysis. The preferential localization of the immunoreaction product seemed to be cytoplasmatic but, in less differentiated cells, nuclear labeling was occasionally visible. Immunoblotting of subcellular fractions confirmed these data. The high degree of evolutionary conservation of p14.5, the considerable upregulation during cellular differentiation and its potential role as a translational inhibitor may reflect an involvement in basic cellular mechanisms, e.g. a differentiation-dependent regulation of protein synthesis in hepatocytes, renal tubular epithelial cells, smooth muscle cells and MNP.