甲壳素二糖,N, N ' -diacetylchitobiose,异化了大肠杆菌和运输/磷酸烯醇丙酮酸:磷酸化的葡糖磷酸转移酶系统。
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Keyhani不,王LX,李YC,罗斯曼
甲壳素二糖,N, N ' -diacetylchitobiose,异化了大肠杆菌和运输/磷酸烯醇丙酮酸:磷酸化的葡糖磷酸转移酶系统。
生物化学杂志。2000年10月20日,275 (42):33084 - 90。
- PubMed ID
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10913117 (在PubMed]
- 文摘
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我们以前报道,野生型菌株大肠杆菌生长的甲壳素二糖N, N ' -diacetylchitobiose (GlcNAc)(2),作为唯一的碳源(Keyhani: O。罗斯曼,s (1997) Proc。国家的。学会科学。美国94年,14367 - 14371)。nonhydrolyzable模拟(GlcNAc)(2)甲基beta-N, N - [(3) H] diacetylthiochitobioside (((3) H) Me-TCB),被用来描述二糖的运输过程中,被发现是由磷酸烯醇丙酮酸:葡糖磷酸转移酶系统(PTS)。这里和附带的文件(Keyhani: O。Boudker, O。罗斯曼,s(2000)生物。化学。275年,33091 - 33101;Keyhani: O。Bacia, K。罗斯曼,s(2000)生物。化学。275年,33102 - 33109; Keyhani, N. O., Rodgers, M., Demeler, B., Hansen, J., and Roseman, S. (2000) J. Biol. Chem. 275, 33110-33115), we report that transport of [(3)H]Me-TCB and (GlcNAc)(2) involves a specific PTS Enzyme II complex, requires Enzyme I and HPr of the PTS, and results in the accumulation of the sugar derivative as a phosphate ester. The phosphoryl group is linked to the C-6 position of the GlcNAc residue at the nonreducing end of the disaccharide. The [(3)H]Me-TCB uptake system was induced only by (GlcNAc)(n), n = 2 or 3. The apparent K(m) of transport was 50-100 micrometer, and effective inhibitors of uptake included (GlcNAc)(n), n = 2 or 3, cellobiose, and other PTS sugars, i.e. glucose and GlcNAc. Presumably the PTS sugars inhibit by competing for PTS components. Kinetic properties of the transport system are described.