Tetranectin,纤溶酶原kringle物4绑定蛋白。克隆和基因表达模式在人类结肠癌。
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组合名称,Albrechtsen R
Tetranectin,纤溶酶原kringle物4绑定蛋白。克隆和基因表达模式在人类结肠癌。
实验室投资。1992年8月,67 (2):253 - 62。
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1354271 (在PubMed]
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背景:Tetranectin是最近发现的蛋白质结合kringle 4 plasminogen地区(Clemmensen我,彼得森LC, Kluft c . 1986欧元;156:327。实验设计:信使rna编码人类tetranectin克隆利用逆转录酶反应中简并引物聚合酶链反应扩增紧随其后。由此产生的聚合酶链反应产物由DNA测序检查并随后用作筛选人类胎盘cDNA探针图书馆。全长cDNA克隆(TET-1)是孤立的,特征,用于北方污点和原位杂交。结果:DNA测序分析显示874碱基对cDNA包含606个碱基对的开放阅读框编码202个氨基酸。一个经典的信号肽是目前从起始蛋氨酸。成熟tetranectin链由181个氨基酸(M (r) = 20169)。3 '非编码区域包含一个聚腺苷酸化信号和26-residue多聚腺苷酸尾。成熟tetranectin链的氨基酸序列预测显示,除了一个氨基酸,完整的身份,通过测序得到的蛋白质(Fuhlendorff J, Clemmensen我Magnusson年代,生物化学1987;26:6757)。 Northern blot of poly A+ revealed a single band of approximately 1 kb. Northern blot analysis of poly A+ isolated from a series of normal human tissues (lung, liver, spleen, kidney, and pancreas) revealed a distinct hybridization band that was especially prominent in the lungs and spleen. No hybridization signal was detected in three carcinoma cell lines examined in parallel. Northern blot analysis of poly A+ RNA isolated from solid tumors revealed a tetranectin specific mRNA band. In situ hybridizations on tissue sections of colon carcinomas and normal colon tissues revealed a strong and distinct hybridization signal of stromal cells in colon carcinomas but not in tumor cells. Only a few stromal cells were labeled in the normal colon. Immunohistochemically, tetranectin was found in a fibrillar-like pattern in the extracellular matrix around the tumor islands and was not detectable in the normal colon stromal tissue. Plasminogen exhibited a similar immunohistochemical staining pattern as tetranectin. CONCLUSIONS: Human tetranectin cDNA comprises 874 base pairs including a 606-base pair open reading frame encoding 202 amino acids including a classical signal peptide. This protein is produced locally by cells of the stromal compartment of tumors and is deposited into the extracellular matrix. Since tetranectin binds to plasminogen we hypothesize that it could function as an anchor and/or reservoir for plasminogen and similar substances that regulate tumor invasion and metastasis as well as tumor angiogenesis.