鸟氨酸代谢在雄性和雌性大鼠肾脏:鸟氨酸转氨酶和精氨酸酶II的线粒体表达。

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Levillain O, Hus-Citharel Garvi年代,Peyrol年代,Reymond我Mutin M,莫雷尔F

鸟氨酸代谢在雄性和雌性大鼠肾脏:鸟氨酸转氨酶和精氨酸酶II的线粒体表达。

杂志肾杂志。2004年4月,286 (4):F727-38。Epub 2004 2月10。

PubMed ID

14871882 (在PubMed

]

文摘

在肾脏,L-ornithine沿着近曲小管重吸收(PCT),基底经由运营商,由精氨酸酶II(暗)。在这里,肾脏代谢的命运L-ornithine雄性和雌性大鼠中进行了分析。肾脏和肾区解剖和用于免疫印迹分析,免疫荧光法和电子显微镜研究。鸟氨酸转氨酶(OAT)和各局部使用特定的抗体。鸟氨酸氧化是由孵化microdissected小管与L - [1-14C]或L -鸟氨酸(U-14C)在研究基质的存在与否。鸟氨酸脱羧酶(ODC) mrna被原位杂交本地化。48-kDa燕麦蛋白是在男性和女性中发现肾脏,但其水平在后者高出四倍。OAT相对分布从表面皮层增加向外髓质达到最高水平。几乎所有的燕麦蛋白质局部皮质和髓近端小管直(分别CPST和OSPST)。在近端小管直(PST),燕麦的蛋白质分布重叠暗生。 No gender difference in AII protein level was found. OAT and AII were colocalized within PST mitochondria. L-[1-14C]ornithine decarboxylation occurred in all tubules, but predominantly in proximal tubules. L-[1-14C]ornithine decarboxylation was enhanced when L-[1-14C]ornithine was given to tubules as the sole substrate. The use of L-[U-14C]ornithine demonstrated the complete oxidation of ornithine. In conclusion, the OAT gene was expressed more in female rat proximal tubules than in male. Because OAT and AII proteins overlapped in PST mitochondria, L-arginine-derived ornithine may be preferentially converted to L-glutamate, as proven by ornithine oxidation. However, the coexpression of ODC, glutamate decarboxylase, and glutamine synthetase in PST suggests that L-ornithine can also be metabolized to putrescine, GABA, and L-glutamine. The fate of L-ornithine may depend on the cellular context.

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药物靶点

药物	目标	类	生物	药理作用	行动
鸟氨酸	Arginase-2,线粒体	蛋白质	人类	未知的	不可用	细节