的splice-isoform vesicle-associated膜蛋白1 (VAMP-1)包含一个线粒体定位信号。
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Isenmann年代,Khew-Goodall Y,赌博J, Vadas M,瓦滕伯格BW
的splice-isoform vesicle-associated膜蛋白1 (VAMP-1)包含一个线粒体定位信号。
细胞杂志。1998;9 (7):1649 - 60。
- PubMed ID
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9658161 (在PubMed]
- 文摘
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筛选图书馆主要来源于人类内皮细胞vesicle-associated的小说揭示了人类对碘氧基苯甲醚膜蛋白1 (VAMP-1),一种蛋白质参与目标和/或运输囊泡的融合目标膜。这部小说我们称为异构体VAMP-1B和指定VAMP-1A前所述对碘氧基苯甲醚。VAMP-1B似乎是另外VAMP-1拼接的形式。类似的老鼠的拼接变体VAMP-1(也称为VAMP-1B)最近报道。五个不同的培养细胞系,从不同的血统,都包含VAMP-1B但很少或根本没有检测到VAMP-1A mRNA,评估PCR。相比之下,大脑信使rna含有VAMP-1A但没有VAMP-1B。VAMP-1B序列编码一种蛋白质相同VAMP-1A除了carboxy-terminal五个氨基酸。carboxy-terminal VAMP-1锚定在囊泡膜的疏水序列。在疏水性VAMP-1A锚后跟一个苏氨酸,carboxy-terminal氨基酸。VAMP-1B预测疏水膜锚是缩短4个氨基酸,和疏水序列是由三个氨基酸,紧随arginine-arginine-aspartic酸。 Transfection of human endothelial cells with epitope-tagged VAMP-1B demonstrated that VAMP-1B was targeted to mitochondria whereas VAMP-1A was localized to the plasma membrane and endosome-like structures. Analysis of C-terminal mutations of VAMP-1B demonstrated that mitochondrial targeting depends both on the addition of positive charge at the C terminus and a shortened hydrophobic membrane anchor. These data suggest that mitochondria may be integrated, at least at a mechanistic level, to the vesicular trafficking pathways that govern protein movement between other organelles of the cell.