克隆和初始表征人类磷脂酶D2 (hPLD2)。调节hPLD2 ADP-ribosylation因素。
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洛佩兹,阿诺RS,兰柏JD
克隆和初始表征人类磷脂酶D2 (hPLD2)。调节hPLD2 ADP-ribosylation因素。
生物化学杂志。1998年5月22日;273 (21):12846 - 52。
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9582313 (在PubMed]
- 文摘
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磷脂酶D(骑士)在内的各种细胞过程包括膜泡运输、呼吸爆发和有丝分裂发生。第一只克隆人类,PLD1激活小gtpase (ARF)和RhoA ADP-ribosylation等因素。啮齿动物PLD2,大约50%相同PLD1最近从小鼠胚胎克隆(科里,W。唱,T。滚,R。Jenco, J。哈蒙德,S。Altshuller, Y。Bar-Sagi D。莫里斯,。,Frohman m(1997)咕咕叫。医学杂志。191 - 201年)和老鼠大脑(Kodaki T。山下先生,美国(1997)生物。272年化学,11408 - 11413)。这里我们描述图书馆B细胞的克隆和表达人类PLD2 (hPLD2)。 The open reading frame is predicted to encode a 933-amino acid protein (Mr of 105,995); this corresponds to the size of the protein expressed in insect cells using recombinant baculovirus. The deduced amino acid sequence shows 53 and 90% identity to hPLD1 and rodent PLD2, respectively. The mRNA for PLD2 was widely distributed in various tissues including peripheral blood leukocytes, and the distribution was distinctly different from that of hPLD1. hPLD1 and hPLD2 both showed a requirement for phosphatidylinositol 4,5-bisphosphate. Both isoforms showed optimal activity at 10-20 mol % phosphatidylcholine in a mixed lipid vesicle system and showed comparable basal activities in the presence of phosphatidylinositol 4,5-bisphosphate. Unexpectedly, ARF-1 stimulated the activity of hPLD2 expressed in insect cells about 2-fold, compared with a 20-fold stimulation of hPLD1 activity. Thus, not only PLD1 but also hPLD2 activity can be positively regulated by both phosphatidylinositol 4,5-bisphosphate and ARF.