PHOSPHO1-A小说磷酸酶明确表示在网站的矿化骨和软骨。
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休斯顿B,斯图尔特AJ, Farquharson C
PHOSPHO1-A小说磷酸酶明确表示在网站的矿化骨和软骨。
骨头。2004年4月,34 (4):629 - 37。
- PubMed ID
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15050893 (在PubMed]
- 文摘
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矿化骨和软骨对骨骼发育和功能至关重要。我们曾报道一种新型基因(PHOSPHO1);的一员大haloacid dehalogenase水解酶超家族,磷酸酶的活性部位指示性。其高表达在骨骼组织让我们推测PHOSPHO1可能参与矿化过程。因此,在这项研究中,我们已经确定,PHOSPHO1本地化网站的软骨和骨矿化。派生PHOSPHO1重组蛋白生产和亲和纯化PHOSPHO1抗血清并用于生成应用一系列的骨骼和软鸟类组织。此外,PHOSPHO1基因表达决心SaOS-2通过rt - pcr和osteoblast-like mg - 63细胞。在皮质骨生长,免疫组织化学本地化PHOSPHO1骨膜的蛋白质类骨质层,形成表面骨单位增长,和新形成的骨细胞,而骨单位骨内膜和关闭都是负面的。在生长板软骨,免疫反应性仅限于Ranvier的早期肥厚性软骨细胞和成骨槽。软骨残余并在主松质骨小梁mineralising表面表现出强烈的免疫反应性。 In 17-day-old embryonic calvaria, the osteoid present on the intramembranous and periosteal bone surfaces stained positively for PHOSPHO1. All soft tissues examined were negative. PHOSPHO1 gene expression was detected in mineralising SaOS-2 but not in the non-mineralising MG-63 osteoblast-like cells and gene expression levels were unchanged by dexamethasone, estradiol, 1,25-dihydroxyvitamin D3 or PTHrP treatment. Western analysis of chick growth plate cell lysate yielded bands (30.4 and 28.6 kD) corresponding to transcripts initiated at each of two possible initiation codons indicating the presence of alternative transcripts for PHOSPHO1 in growth cartilage. These results confirm that the PHOSPHO1 protein and gene expression profile is consistent with a role for PHOSPHO1 in bone and cartilage matrix mineralisation.