ATP-dependent 6-phosphofructokinase

Details

Name
ATP-dependent 6-phosphofructokinase
Synonyms
  • 2.7.1.11
  • ATP-PFK
  • pfk
  • Phosphohexokinase
Gene Name
pfkA
Organism
Geobacillus stearothermophilus
Amino acid sequence
>lcl|BSEQ0019188|ATP-dependent 6-phosphofructokinase MKRIGVLTSGGDSPGMNAAIRSVVRKAIYHGVEVYGVYHGYAGLIAGNIKKLEVGDVGDI IHRGGTILYTARCPEFKTEEGQKKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEHGFPCV GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWS GLAGGAETILIPEADYDMNDVIARLKRGHERGKKHSIIIVAEGVGSGVDFGRQIQEATGF ETRVTVLGHVQRGGSPTAFDRVLASRLGARAVELLLEGKGGRCVGIQNNQLVDHDIAEAL ANKHTIDQRMYALSKELSI
Number of residues
319
分子量
34118.62
Theoretical pI
6.96
GO Classification
Functions
6-phosphofructokinase activity/ATP binding/metal ion binding
Processes
fructose 6-phosphate metabolic process
Components
cytoplasm
General Function
Metal ion binding
Specific Function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0003601|960 bp GTGAAACGCATTGGTGTGTTGACAAGCGGCGGCGACTCGCCGGGAATGAATGCGGCCATT CGCTCGGTTGTCCGCAAAGCGATTTACCACGGTGTGGAAGTGTACGGCGTTTATCACGGG TATGCCGGACTGATCGCCGGCAACATTAAAAAGCTGGAAGTCGGCGATGTCGGGGATATT ATCCACCGTGGCGGCACGATACTCTATACGGCCCGCTGTCCGGAATTTAAGACGGAAGAA GGTCAGAAAAAAGGGATCGAGCAGCTGAAAAAGCACGGCATTGAAGGGCTGGTCGTCATC GGCGGCGACGGTTCGTACCAAGGGGCGAAAAAACTGACCGAGCACGGCTTTCCGTGCGTC GGCGTGCCGGGGACGATTGACAACGACATTCCGGGCACTGATTTTACGATCGGGTTTGAC ACGGCGCTCAATACGGTCATTGATGCCATCGACAAAATCCGCGACACGGCGACGTCGCAC GAGCGGACGTACGTCATCGAAGTGATGGGCCGCCATGCCGGCGACATCGCCTTATGGTCG GGGCTGGCCGGCGGGGCGGAAACGATTTTAATTCCGGAAGCCGATTATGATATGAACGAC GTCATCGCCCGCTTAAAGCGCGGCCATGAGCGCGGCAAAAAACATAGCATCATCATCGTG GCGGAAGGAGTTGGAAGCGGCGTCGACTTCGGCCGGCAAATCCAGGAAGCGACCGGCTTT GAGACGCGTGTGACGGTGCTTGGCCACGTGCAGCGCGGCGGGTCGCCCACGGCGTTTGAC CGCGTGCTCGCGAGCCGCCTCGGCGCCCGGGCGGTCGAGCTGTTGCTCGAAGGAAAAGGC GGGCGCTGCGTCGGCATCCAAAACAACCAGCTCGTCGACCATGACATCGCGGAGGCGCTA GCCAACAAACATACGATTGATCAGCGGATGTACGCATTGTCGAAAGAATTGTCCATTTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
Resource Link
UniProtKB ID P00512
UniProtKB Entry Name PFKA_GEOSE
GenBank Protein ID 143312
GenBank Gene ID M15643
General References
  1. French BA, Chang SH: Nucleotide sequence of the phosphofructokinase gene from Bacillus stearothermophilus and comparison with the homologous Escherichia coli gene. Gene. 1987;54(1):65-71. [Article]
  2. Kolb E, Hudson PJ, Harris JI: Phosphofructokinase: complete amino-acid sequence of the enzyme from Bacillus stearothermophilus. Eur J Biochem. 1980 Jul;108(2):587-97. [Article]
  3. Sakai H, Ohta T: Molecular cloning and nucleotide sequence of the gene for pyruvate kinase of Bacillus stearothermophilus and the production of the enzyme in Escherichia coli. Evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. Eur J Biochem. 1993 Feb 1;211(3):851-9. [Article]
  4. Byrnes M, Zhu X, Younathan ES, Chang SH: Kinetic characteristics of phosphofructokinase from Bacillus stearothermophilus: MgATP nonallosterically inhibits the enzyme. Biochemistry. 1994 Mar 22;33(11):3424-31. [Article]
  5. 埃文斯公关,哈德逊PJ:结构和无机磷的控制phofructokinase from Bacillus stearothermophilus. Nature. 1979 Jun 7;279(5713):500-4. [Article]
  6. Evans PR, Farrants GW, Hudson PJ: Phosphofructokinase: structure and control. Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):53-62. [Article]
  7. Schirmer T, Evans PR: Structural basis of the allosteric behaviour of phosphofructokinase. Nature. 1990 Jan 11;343(6254):140-5. [Article]
  8. Riley-Lovingshimer MR, Ronning DR, Sacchettini JC, Reinhart GD: Reversible ligand-induced dissociation of a tryptophan-shift mutant of phosphofructokinase from Bacillus stearothermophilus. Biochemistry. 2002 Oct 29;41(43):12967-74. [Article]
  9. Mosser R, Reddy MC, Bruning JB, Sacchettini JC, Reinhart GD: Structure of the apo form of Bacillus stearothermophilus phosphofructokinase. Biochemistry. 2012 Jan 24;51(3):769-75. doi: 10.1021/bi201548p. Epub 2012 Jan 11. [Article]
  10. Mosser R, Reddy MC, Bruning JB, Sacchettini JC, Reinhart GD: Redefining the role of the quaternary shift in Bacillus stearothermophilus phosphofructokinase. Biochemistry. 2013 Aug 13;52(32):5421-9. doi: 10.1021/bi4002503. Epub 2013 Jul 31. [Article]

Drug Relations

Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details
DB02726 2-Phosphoglycolic Acid experimental unknown Details
DB04493 Fructose-6-phosphate experimental unknown Details
DB09344 Invert sugar experimental no substrate Details