DNA-3-methyladenine glycosylase 1
Details
- Name
- DNA-3-methyladenine glycosylase 1
- Synonyms
-
- 3-methyladenine-DNA glycosylase I, constitutive
- 3.2.2.20
- DNA-3-methyladenine glycosidase I
- DNA-3-methyladenine glycosylase I
- TAG I
- Gene Name
- tag
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
-
>lcl|BSEQ0011178|DNA-3-methyladenine glycosylase 1 MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGLSWITVLKKRENYRACF HQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAYLQMEQNGEPFVDFVWSFV NHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTTICYSFMQACGLVNDHVVGCC CYPGNKP
- Number of residues
- 187
- 分子量
- 21099.98
- Theoretical pI
- 6.65
- GO Classification
-
FunctionsDNA-3-methyladenine glycosylase activity/metal ion bindingProcessesbase-excision repair
- General Function
- Metal ion binding
- Specific Function
- Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions.
- Pfam Domain Function
-
- Adenine_glyco (PF03352)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
-
>lcl|BSEQ0011179|DNA-3-methyladenine glycosylase 1 (tag) ATGGAACGTTGCGGCTGGGTGAGTCAGGACCCGCTTTATATTGCCTACCATGATAATGAG TGGGGCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAG CAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTT CATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTA CAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGG GCGTACCTGCAAATGGAACAGAACGGCGAACCGTTTGTCGACTTTGTCTGGTCGTTTGTA AATCATCAGCCACAGGTGACACAAGCCACAACGTTGAGCGAAATTCCCACATCTACGTCC GCCTCCGACGCCCTATCTAAGGCACTGAAAAAACGTGGTTTTAAGTTTGTCGGCACCACA ATCTGTTACTCCTTTATGCAGGCATGTGGGCTGGTGAATGATCATGTGGTTGGCTGCTGT TGCTATCCGGGAAATAAACCATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
-
Resource Link UniProtKB ID P05100 UniProtKB Entry Name 3MG1_ECOLI GenBank Protein ID 147920 GenBank Gene ID J02606 - General References
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- Sakumi K, Nakabeppu Y, Yamamoto Y, Kawabata S, Iwanaga S, Sekiguchi M: Purification and structure of 3-methyladenine-DNA glycosylase I of Escherichia coli. J Biol Chem. 1986 Nov 25;261(33):15761-6. [Article]
- Steinum AL, Seeberg E: Nucleotide sequence of the tag gene from Escherichia coli. Nucleic Acids Res. 1986 May 12;14(9):3763-72. [Article]
- Sofia HJ, Burland V, Daniels DL, Plunkett G 3rd, Blattner FR: Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res. 1994 Jul 11;22(13):2576-86. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
- Drohat AC, Kwon K, Krosky DJ, Stivers JT: 3-Methyladenine DNA glycosylase I is an unexpected helix-hairpin-helix superfamily member. Nat Struct Biol. 2002 Sep;9(9):659-64. [Article]
- Kwon K, Cao C, Stivers JT: A novel zinc snap motif conveys structural stability to 3-methyladenine DNA glycosylase I. J Biol Chem. 2003 May 23;278(21):19442-6. Epub 2003 Mar 24. [Article]
- Cao C, Kwon K, Jiang YL, Drohat AC, Stivers JT: Solution structure and base perturbation studies reveal a novel mode of alkylated base recognition by 3-methyladenine DNA glycosylase I. J Biol Chem. 2003 Nov 28;278(48):48012-20. Epub 2003 Sep 16. [Article]